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hillary duff
hillary duff asked in Science & MathematicsChemistry · 1 decade ago

helppppppppppppppppppp?

1. masses of solid tris and tris HCl( Tris= tris(hydroxymethyl)aminomthane) should be combined and diluted to 100mL to make a 0.100M buffer with ph = 7.40?

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  • Xartyve2
    1 decade ago
    Favorite Answer

    Help! I need somebody! Help! Not just anybody! Help! You know I need someone! Heeelp!

  • Old Science Guy
    Lv 7
    1 decade ago

    check out 15. on page 7 of this reference

    http://2008.igem.org/wiki/images/8/84/Protein_Buff...

    you could work backwards from this chart to get appropriate masses for your volumes

    I am totally unfamiliar with the ionization of tris

    but

    I did find a pKa of 8.06 giving a Ka of 8.17E-9

    If it were essentially monoprotic the math would be fairly straight forward

    but, again, not knowing I cannot say

    Sorry for the minimal help

    and it's quite possible that I misunderstood the whole question

     

     

     

     

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