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Undergrad knowledge needed help please?
In column chromatography we are using an enert gel to seperate monosacharides from starch based up on their size. The solvent is a NaCL solution
What role does the NaCL play??
Something about ionization effect, yet i look that up and it says the ions would interact with charged portions of the substrate..... glucose and starch are not charged yet they have a dipole?? soooo would these ions interact with the dipoles so that they dont interact with each other and flow out better ?? if so....i dont understand how they would react?? the NA+ or the Cl+ or both??
Cl - not Cl+ sorry
The sample is a mix of monosacharides and polysaccharides, they will seperated by their size with, Lugol is used to detect startch (helical coils) and benedicts is used to detect glucose and other reducing sugars
1 Answer
- Evolv589Lv 41 decade agoFavorite Answer
The NaCl is used to "solvate" the molecules, that is - it will interact with polar components of the monosaccharides and keep them from interacting with each other. In other words, it will keep them separated.
What I'm confused about is that starch is a polymer of glucose, so I'm not sure what would be separated.
