Angela Arriola

The protein concentration of a protein isolate was determined using the Bradford assay. Five dilution of bovine serum albumin (BSA) were prepared from a 2.00 mg/mL BSA stock solution. To these dilutions, 100 µL of Bradford reagent were added. After 5 minutes, the absorbance was taken at 595 nm. 

The supernatant dilution was prepared by mixing 10 µL of the protein isolate with 20 µL water. The absorbance was taken 5 minutes after addition of 100 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original isolate if the absorbance at 595 nm was 0.399.

 A unique aquatic plant was discovered from a lagoon in El Nido, Palawan. To determine the protein content of the plant, an adequate amount of the plant extract was acquired, and Bradford assay was performed. Determine the total protein concentration, in μg/mL, of the acquired plant extract. A bovine serum albumin (BSA) stock solution with a concentration of 500 μg/mL was used and mixtures with the following compositions and absorbance readings were prepared: